H7415
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 | Immunoblotting Cell lysates of HEK-293T overexpressing human huntingtin N-terminal fragment (Lanes 1,3) and HEK-293T non-transfected (Lane 2), were separated on SDS-PAGE, blotted with Anti-Huntingtin (N-terminal region) (Cat. No. H7415) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 0.25 μg/mL 2. Antibody: 0.25 μg/mL 3. Antibody: 0.25 μg/mL + huntingtin immunizing peptide (human, 64-82) |
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H7415
Sigma
Anti-Huntingtin (N-terminal region) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-HD, Anti-HTT, Anti-Huntington disease |
Description
| Immunogen | synthetic peptide corresponding to amino acids 64-82 of human huntingtin, conjugated to KLH. This sequence is identical in rat and mouse huntingtin. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~110 kDa |
| species reactivity | human |
| concentration | ~1.5 mg/mL |
| application(s) | immunoblotting: 0.25-0.5 μg/mL using HEK-293T cell lysate expressing a N-terminal fragment of human huntingtin |
| Gene Information | human ... HTT(3064) |
| rat ... Htt(29424) | |
| mouse ... Htt(15194) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 1 |
Related Products
| Related product | H7540, Anti-Huntingtin (N-terminal) antibody produced in rabbit |
